The untreated SEB was stable throughout experimentation. For each sample, the individual CD scans were compared prior to averaging to ascertain the structural stability of the sample. The protection of water supplies and water distribution infrastructure such as reservoirs is of great concern for those defending against bioterrorism. The percentages reported here were obtained using the Reed reference set for the secondary structure calculations [ 9 ]. After 15—20 minutes, the pH was assessed and adjusted if needed with minimal volume change to 7. RO water was poured into the same filter unit, filtered, and collected.
Depending on which manufacturer of HHA is being tested, the technology can be low cost, requires no electrical power, and requires minimal to no sample preparation to test a sample.
International Journal of Microbiology
Gargan II, and W. Chlorinated water was considered stable for only one day and therefore made fresh daily just prior to use. Both disinfectants are extremely effective sanitizers, killing microbes in minutes. Additionally, it was noted in the present study that chlorine did not impact detection levels of ricin as significantly as detection levels of SEB under the test conditions employed. Ricin and SEB are considered potential biological threat agents as both can cause illness either by inhalation or ingestion. The CD and PAGE results together suggest a major change in SEB structure that may result in multiple SEB-based antigens, the majority of which are less easily recognized and not bound as effectively as untreated SEB by the antibody present on the membrane of the assay. Therefore, it is likely that protein toxins would also be affected by these strong oxidants.